Zebrafish cx30.3: identification and characterization of a gap junction gene highly expressed in the skin.

We have identified and characterized a zebrafish connexin, Cx30.3. Sequence similarity analyses suggested that Cx30.

Tandem alternative splicing of zebrafish connexin45.6.

Early studies suggested that most connexin genes share a relatively simple structure with a single intron of variable length interrupting the 5' untranslated region (UTR). Here we report that zebrafish cx45.6 shows six isoforms of alternative 5'UTRs which are generated from multiple promoter usage and alternative pre-mRNA splicing.

Posttranslational modifications in lens fiber connexins identified by off-line-HPLC MALDI-quadrupole time-of-flight mass spectrometry.

Purpose: Gap junction intercellular communication is necessary for the development and maintenance of the lens. Lens fiber connexins are known to be posttranslationally modified, but little detail is available regarding the nature of some of these modifications and the specific amino acids affected. The purpose of this study was to identify posttranslational modification in the bovine lens fiber connexins, Cx44 and Cx49.

Developmental regulation and expression of the zebrafish connexin43 gene.

We cloned and sequenced the zebrafish (Danio rerio) connexin43 (Cx43alpha1) gene. The predicted protein sequence shows a high degree of sequence conservation. Transcript analyses revealed multiple transcription start sites and a potential alternative transcript encoding a N-terminally truncated Cx43alpha1 protein.