[Use of PCR for detection of maize seeds infected with the fungus Fusarium moniliforme Sheldon var.lactis].

The procedure of the PCR for the detection of Fusarium moniliforme Sheldon var. lactis on the maize seeds has been elaborated. The based on a highly sensitive molecular genetics technology method of fungal pathogen detection allows to reveal its presence before the visual symptoms of seed damage.

[Increased selectivity of directed RNA hydrolysis mediated by RNAse H from E. coli using mixed oligo(deoxyribo-ribo)nucleotides].

Chimeric oligo(deoxyribo-ribo)nucleotides appeared to be a valuable tool to achieve the high selectivity of RNA cleavage as shown by RNA-ase H-mediated hydrolysis of TMV RNA directed by d(TGTGTATGCC), d(TGTGTAT), d(TGTGTAT)GCCAU and d(TGTGTAT)ppGCCAU.

[Chemico-enzymatic method of synthesis of mixed oligonucleotides].

Oligo (ribodeoxyribo)nucleotide d(TGTGTAT)r(GCCAU) was synthesized from oligonucleotide blocks by chemical ligation, the oligoribonucleotide and oligodeoxynucleotide blocks being prepared by enzymatic and chemical synthesis, resp. A general procedure of the hybride duplex strand separation is described.