[Pulmonary surfactant in guinea pigs after toxic exposure to CCl4].

The surface-active properties of lung surfactant were studied in 32 guinea pigs given CCl4 according to a schedule. The surface activity of the surfactant is inhibited in the experimental animals under the effect of hepatotrophic poison (CCl4) due to the disturbed metabolism of its phospholipids, which is a consequence of the diminished lipid-synthesizing function of the liver. Diminution of the surface-active properties of the lung surfactant and disturbance of its qualitative composition may be regarded as the cause of the development of extensive atelectases in the animals' lungs.

[A method of isoelectric focusing in a borate-polyol system with pH gradient of 4.0 to 5.6 in a thin layer of polyacrylamide gel].

A procedure is described for thin-layer polyacrylamide gel isoelectric focusing involving borate-polyol system with gradient pH 4.0-5.6, where 0.

[Two-dimensional electrophoresis of the products of limited hydrolysis of human serum albumin using isoelectric focusing in a borate-polyol system].

A procedure developed involved isoelectrofocusing in borate-polyol system and thin-layer electrophoresis in polyacrylamide gel gradient containing sodium dodecylsulfate of the products of human blood serum albumin obtained after partial hydrolysis. The first dimension isoelectrofocusing in borate-polyol system allowed to separate peptides with close values of pI and to increase the procedure sensitivity. The procedure may be recommended for identification of proteins, for estimation of the protein degradation in hydrolysis as well as for studies of peptide maps of albumin under normal and pathological states.