Exosome-mediated delivery of CRISPR-Cas9: A revolutionary approach to cancer gene editing.

Several molecular strategies based on targeted gene delivery systems have been developed in recent years; however, the CRISPR-Cas9 technology introduced a new era of targeted gene editing, precisely modifying oncogenes, tumor suppressor genes, and other regulatory genes involved in carcinogenesis. However, efficiently and safely delivering CRISPR-Cas9 to cancer cells across the cell membrane and the nucleus is still challenging. Using viral vectors and nanoparticles presents issues of immunogenicity, off-target effects, and low targeting affinity.

A coumarin and 1,8-napthyridine conjugated novel molecular hybrid: Synthesis, DFT insights and unveiling the selective fluorescent sensing of Hg ions with live-cell imaging application.

Herein, we have used a simple synthetic strategy to access a novel non-sulfur fluorescent molecular probe coumarin and 1,8-napthyridine conjugated probe DNCS. The developed probe has great selectivity and sensitivity for detecting Hg ions. Our photophysical properties evaluation for the synthesized probe with different metal ions (Ba, Al, Ca, Bi, Ce, Cd, Cu, Sr, Co, Fe, Cr, Fe, Mn, Hg, Zn, Pb, Ni, and Sn) unveiled the very selective and sensitive fluorescence sensing behavior with Hg ions in the energy window of near UV and visible light radiation in an organic aqueous solvent mixture (EtOH and water).

The Impact of Thoracic Trauma on Morbidity and Outcomes: A Six-Year Experience From a Tertiary Care Level 1 Center.

Objective: The study aimed to summarize the impact of thoracic injury on mortality and morbidity in thoracic trauma patients.

Material And Methods: This is a retrospective observational study of all patients with thoracic injuries admitted between July 2018 and June 2024. The demographic profile, mechanism of injury, type of injury, injury severity score, duration of injury, hemodynamic status, surgical findings, concomitant injuries, hospitalization, intensive care unit stay, need for mechanical ventilation, and blood transfusions were recorded.

Prostate cancer burden in South Asia: A systematic analysis of global burden of disease data (1990-2021).

Objectives: The objectives of this study were to analyze trends in prostate cancer incidence, incidence, mortality, and disability-adjusted life years (DALYs) from 1990 to 2021 via data from the Global Burden of Disease (GBD) study in South Asia. Additionally, the study projects future prostate cancer incidence rates up to 2031 to inform public health interventions in South Asia.

Methods: Data covering South Asian countries such as Bangladesh, Bhutan, India, Nepal, and Pakistan were obtained from the GBD 2021 portal.