[Lipid Blood Profile in Old Patients With Ischemic Heart Disease].

Purpose: to assess prevalence of dyslipidemia in patients with ischemic heart disease (IHD) older than 75 years as well as to evaluate possible associations between serum lipids and various cardiovascular and other diseases in these patients.

Methods: We enrolled in this cross sectional study 555 hospitalized IHD patients aged 75-98 years (mean age 86.8 years, 74.

[Monoclonal antibodies to insulin influencing its biological activity].

Somatic hybridization was employed for obtaining 335 hybridomas producing monoclonal antibodies to insulin. Twelve hybridomas were cloned by a method of maximum dilutions, and specific immunoglobulins, secreted by them, were characterized by solid phase immunoenzyme assay. Monoclonal antibodies possessed different activity with relation to human, porcine and cattle insulins, indicating their specificity to different epitopes on the insulin molecule.

[3H-cortisol binding with hormone-sensitive and hormone-resistant thymocytes].

A study was made of 3H-cortisol binding with total, hormone-sensitive and hormone-resistant populations of adrenalectomized rat thymocytes before and after their division using BCA and percoll gradients. Subpopulations of small and large thymocytes obtained by using percoll were hormone resistant and hormone sensitive, respectively. The method of thymocyte division on percoll gradient was effective for a study of thymocyte interaction with glucocorticoids.

[Effect of polymer-immobilized cortisol on 3H-uridine incorporation into the thymocyte RNA of rats].

It was found that cortisol immobilized on polyvinylpyrrolidone (PVP-GC) suppresses the incorporation of 3H-uridine into RNA of thymocytes of intact but not adrenalectomized rats. PVP-GC at the concentration of 10(-6) M enhanced the inhibiting effect of dexamethasone (10(-9) M) and cortisol (10(-9) M) on RNA biosynthesis in thymocytes of adrenalectomized animals.