Neonatal Exposure to Bacterial Lipopolysaccharide Affects Behavior and Expression of Ionotropic Glutamate Receptors in the Hippocampus of Adult Rats after Psychogenic Trauma.

According to the two-hit hypothesis of psychoneuropathology formation, infectious diseases and other pathological conditions occurring during the critical periods of early ontogenesis disrupt normal brain development and increase its susceptibility to stress experienced in adolescence and adulthood. It is believed that these disorders are associated with changes in the functional activity of the glutamatergic system in the hippocampus. Here, we studied expression of NMDA (GluN1, GluN2a, GluN2b) and AMPA (GluA1, GluA2) glutamate receptor subunits, as well as glutamate transporter EAAT2, in the ventral and dorsal regions of the hippocampus of rats injected with LPS during the third postnatal week and then subjected to predator stress (contact with a python) in adulthood.

[PROSPECTS OF THE NUCLEAR PROTEIN NeuN APPLICATION AS AN INDEX OF FUNCTIONAL STATE OF THE VERTEBRATE NERVE CELLS].

Assessment of safety, viability, and functional state of nerve cells is the major problem in studies of experimental effects on various structures of vertebrate brain and in search for correlation between structural abnormalities and changes in physiological parameters. Such an assessment is possible with applying an immunocytochemical reaction to neuronal nuclear antigen NeuN discovered in 1992. Numerous studies of the protein showed its neural specificity and its amino acid consequence was found to have high interspecies concervatism.

[CYTOPLASMIC LOCALIZATION OF NEUN/Fox-3 NUCLEAR PROTEIN IN THE PANCREATIC CELLS].

The study of the sections of Wistar rat pancreas (n=4) using the methods of immunocytochemistry and confocal laser microscopy has demonstrated previously unknown fact of the presence of NeuN protein in the secretory epithelial cells of the exocrine part of this organ. The protein was located in the apical part of the cytoplasm slightly below the zone of zymogen granules. Until now the nuclear protein NeuN was considered to be the panneuronal differentiation marker and was used in the studies of neurogenesis.

[Method of selective demonstration of proliferating cells in the structures of developing nervous system based on the detection of phosphorylated H3 histone].

Phosphorylated H3 histone is of interest for the study of proliferative activity of the cells in various tissues. Its detection is used in histopathological tumor diagnosis. The purpose of this study was to develop immunocytochemical protocols for the demonstration of H3 phosphohistone in the cells of the developing nervous system for classical light microscopy and confocal laser microscopy.

[Proliferation markers used in histological studies].

The present paper reviews the current approaches used to study cell proliferative activity. In the last years, a number of proteins involved in cell cycle control were discovered, that may serve as selective markers of proliferating cells. This work gives the characteristics of immunocytochemical methods demonstrating 5-bromodeoxyuridine, PCNA, Ki-67, FEN-1, phosphorylated histone H3 and cyclins.