Publications by authors named "G Terzioglu"

Reactive astrocytes are associated with Alzheimer's disease (AD), and several AD genetic risk variants are associated with genes highly expressed in astrocytes. However, the contribution of genetic risk within astrocytes to cellular processes relevant to the pathogenesis of AD remains ill-defined. Here, we present a resource for studying AD genetic risk in astrocytes using a large collection of induced pluripotent stem cell (iPSC) lines from deeply phenotyped individuals with a range of neuropathological and cognitive outcomes.

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Article Synopsis
  • Reactive astrocytes are linked to Alzheimer’s disease (AD), and this study explores how genetic risk factors in these cells contribute to AD pathology using induced pluripotent stem cells (iPSCs) from individuals with varying cognitive outcomes.
  • The research involves creating astrocytes from iPSCs of 44 individuals and employing RNA sequencing and mass spectrometry to profile molecular features, revealing consistent dysregulation in genes and pathways between iPSC-derived astrocytes and actual AD brain tissue.
  • Notable findings include differences in protein levels related to cellular respiration and interferon responses, indicating that higher genetic risk for AD correlates with lower levels of protective interferon response proteins, and suggesting a new experimental framework for studying genetic influences on
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Recent genetic studies on Alzheimer's disease (AD) have brought microglia under the spotlight, as loci associated with AD risk are enriched in genes expressed in microglia. Several of these genes have been recognized for their central roles in microglial functions. Increasing evidence suggests that SHIP1, the protein encoded by the AD-associated gene INPP5D, is an important regulator of microglial phagocytosis and immune response.

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Microglia and neuroinflammation play an important role in the development and progression of Alzheimer's disease (AD). Inositol polyphosphate-5-phosphatase D (INPP5D/SHIP1) is a myeloid-expressed gene genetically-associated with AD. Through unbiased analyses of RNA and protein profiles in INPP5D-disrupted iPSC-derived human microglia, we find that reduction in INPP5D activity is associated with molecular profiles consistent with disrupted autophagy and inflammasome activation.

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Background/aim: Serum and growth factor deprivation, a common cellular stressor in solid tumors, arises upon irradiation, chemotherapy, and antiangiogenesis. Spheroid body culture aims to enrich cancer stem cells by using low attachment conditions and some growth factors, such as basic fibroblast growth factor and epidermal growth factor to support the spheroid formation in serum-free spheroid culture. However, spheroid culture without any growth factors can imitate the tumor environment more realistically.

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