RELIABILITY AND VALIDITY OF THE KAZAKH-LANGUAGE ACT QUESTIONNAIRE AS AN ASTHMA CONTROL TOOL.

Background: To date, several questionnaires have been developed as easy-to-use screening tools for assessing and monitoring asthma control as the Asthma Control Test (ACT). However, the assessment of the reliability of the ACT questionnaire translated into the Kazakh language has not been carried out yet. The study aimed to evaluate the reliability and validity of the Kazakh-language ACT questionnaire as an asthma control tool.

[Cytochemical parameters in studying the body at peripheral hemopoiesis level].

The authors used cytochemical methods to study functions of neutrophils, RBC and lymphocytes of perpheral blood in miners suffering from anthracosilicosis stage I, revealed higher phospholipids content, lower catecholamines, increased activity of monoamine oxidase, esterase, decreased glycogen, RNA and lysosomal cationic proteins, in comparison with apparently healthy individuals. These tests are considered expedient in diagnosis of early anthracosilicosis manifestations in miners.

[The histochemical detection of insulin in a culture of pancreatic endocrine tissue by using the pseudoisocyanine and immunofluorescence methods].

Histochemical pseudoisocyanin and immunofluorescent methods were employed to investigate the level of insulin in cultivated pancreatic islet tissue (PIT) using two techniques: 1) staining of isolated PIT, cultivated on mica sheets; 2) staining of cultivated PIT sections. Stained sections ensure better results of microscopic and photometric investigations. On the whole, the most optimum method for histochemical analysis of the state of cultivated PIT is the combination of the above two techniques.

[A histofluorimetric method of evaluating the insulin level of pancreatic islets].

Up to now results of the histochemical luminiscent reaction to insulin in pancreatic islet beta cells (the pseudoisocyanin test) were assessed by a visual method which permitted establishing the difference only in substantial variations of fluorescence intensity. The authors employed a quantitative method for assessment of the results of the test using a microfluorimetric attachment designed by them. They also provided data on the measurement of the insulin content in pancreatic islet beta cells of rats in different experimental exposures using this method.