[Immunomodulating properties of khlotazol].

A nonsteroidal anti-inflammatory agent khlotazol administered to rats in doses of 40-80 mg/kg for 5 days stimulated synthesis of IgM- and IgG-antibodies to ram erythrocytes and increased the number of antibody-forming cells (AFC) and rosette-forming cells (RFC) in the spleen. When the doses were increased to 160-320 mg/kg, the stimulating effect of the drug on the production of hemagglutinins retained but dose-dependent inhibition of the number of AFC and RFC was found. When administered in a dose of 80 mg/kg khlotazol markedly activated the reaction of hypersensitivity of delayed type induced by ram erythrocytes.

[Antibody formation during use of heterologous trypsin].

A five-fold administration to Wistar rats of crystalline bovine trypsin in a dose of 0.01 mg per 100 g of body weight stimulated formation of rosette forming cells (RFC) and antibody forming cells (AFC) in the spleen in injection of the antigen 2 hours after the termination of trypsin treatment, but failed to influence the immune response in case of immunization 48 hours after the terminal injection of the enzyme. The blood serum of rats, obtained 2 hours after the last trypsin injection stimulated in passive homologous transfer the RFC and the AFC formation and also accelerated tha antibody production.

[Formation of complete and incomplete antibodies by rabbits immunized with sheep erythrocytes].

A parellel determination of incomplete and complete antibodies in rabbits immunized by various doses of sheep erythrocytes demonstrated that reduction of the titres of complete antibodies occurred more rapidly than that of imcomplete ones. Complete 7S-antibodies appeared in the blood of animals immunized with high doses of erythrocytes or after a repeated injection of low antigen doses. Incomplete 7S-antibodies are determined in the blood of rabbits after a primary immunization with high and with low doses of sheep erythrocytes, The titres of incomplete hemagglutinins were almost the same in the reaction with crude and trypsinized erythrocytes.