Bridging the Gap from Rural Trauma to Rural Healthcare: Fire Department Education Sessions.

South Dakota's Emergency Medical Services (EMS) volunteer participation is declining, and projected to further decrease over the next decade. To minimize this deficit, South Dakota's Department of Health recommends that rural firefighters assist EMS in high-acuity calls to decrease the burden on EMS personnel in an effort to improve volunteer retention. Bridging the Gap from Rural Trauma to Rural Healthcare aimed to create educational training opportunities for firefighters when assisting EMS.

H3K56 acetylation regulates chromatin maturation following DNA replication.

Following DNA replication, the newly reassembled chromatin is disorganized and must mature to its steady state to maintain both genome and epigenome integrity. However, the regulatory mechanisms governing this critical process remain poorly understood. Here, we show that histone H3K56 acetylation (H3K56ac), a mark on newly-synthesized H3, facilitates the remodeling of disorganized nucleosomes in nascent chromatin, and its removal at the subsequent G2/M phase of the cell cycle marks the completion of chromatin maturation.

Regulating IL-2 Immune Signaling Function Via A Core Allosteric Structural Network.

Human interleukin-2 (IL-2) is a crucial cytokine for T cell regulation, with therapeutic potential in cancer and autoimmune diseases. However, IL-2's pleiotropic effects across different immune cell types often lead to toxicity and limited efficacy. Previous efforts to enhance IL-2's therapeutic profile have focused on modifying its receptor binding sites.

Accounting for Fast vs Slow Exchange in Single Molecule FRET Experiments Reveals Hidden Conformational States.

Proteins are dynamic systems whose structural preferences determine their function. Unfortunately, building atomically detailed models of protein structural ensembles remains challenging, limiting our understanding of the relationships between sequence, structure, and function. Combining single molecule Förster resonance energy transfer (smFRET) experiments with molecular dynamics simulations could provide experimentally grounded, all-atom models of a protein's structural ensemble.