Publications by authors named "G Styba"
Article Synopsis
- The COVID-19 pandemic emphasized the need for reliable and scalable sources of viral antigens for research and diagnostic purposes.
- Researchers adapted microalgae (Phaeodactylum tricornutum) to create an inducible system to overexpress the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein, improving efficiency and yield.
- The produced RBD was effective in diagnostics, demonstrating similar sensitivity to traditional assays, indicating that microalgae could serve as a viable platform for antibody production in pandemic responses.
Carbonic anhydrase III (CA III) is an enzyme present in skeletal muscle which is released into circulation following injury. Myoglobin (Mb) is a heme protein located in skeletal, smooth, and cardiac muscle which is also released after injury. Because CA III is not present in myocardium, combining serum CA III and Mb measurements may improve the specificity of Mb as an early diagnostic marker for myocardial infarction (MI) provided that a fixed ratio of Mb and CA III is released from skeletal muscle following cell injury.
View Article and Find Full Text PDFObjectives: To determine the serum and plasma level of human cardiac troponin I (cTnI) resulting from myocardial damage, we have developed a sensitive and specific one-step enzyme immunoassay to measure cardiac troponin I.
Design And Methods: The COBAS cTnI assay is a semi-automated one-step solid phase immunoassay compatible with the COBAS Core. The assay is performed in a sandwich type format using a polyclonal goat antibody capture and two highly specific horseradish peroxidase conjugated monoclonal antibody detectors directed against different epitopes of the cTnI molecule.
Objectives: To develop an effective method to remove endotoxin from large scale E. coli recombinant protein purifications.
Design And Methods: Triton X-114 phase separation, affinity chromatography utilizing immobilized polymyxin B or immobilized histidine, were used to remove endotoxin from purified preparations of recombinant CK-BB, CK-MB, CK-MM, myoglobin, and cardiac troponin I.