First efficacy and safety study of femtosecond lenticule extraction for the correction of myopia: six-month results.

Purpose: To prospectively study the feasibility of femtosecond lenticule extraction (FLE), a new method of refractive correction.

Setting: Department of Ophthalmology, Philipps University of Marburg and Helios Clinic, Erfurt, Germany.

Methods: A flap and a lenticule of intrastromal corneal tissue were simultaneously cut with a VisuMax femtosecond laser system.

Two-photon excitation and emission spectra of the green fluorescent protein variants ECFP, EGFP and EYFP.

Summary Two-photon (TP) excitation (820-1150 nm) and emission (280-700 nm) spectra for the fluorescent proteins (FPs) ECFP3, EGFP3 and EYFP3 produced in human tumour cells were recorded. TP excitation spectra of pure and highly enriched samples were found to be more differentiated in comparison with their one-photon (OP) spectra. They exhibited more pronounced main and local maxima, which coincided among different purity grades within small limits.

Femtosecond-pulse sequence compression by Gires-Tournois interferometers.

We demonstrate the compression of femtosecond-pulse sequences by phase-modulating resonators, such as Gires-Tournois interferometers. The experiments are based on the precompensation of the complex phase response of the resonator by a high-resolution liquid-crystal pulse shaper. This method can be utilized for lowering peak intensities at critical points in optical setups, as well as for encryption or decryption of ultra-short pulses.

Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging.

Two-photon absorption and emission spectra for fluorophores relevant in cell imaging were measured using a 45 fs Ti:sapphire laser, a continuously tuneable optical parametric amplifier for the excitation range 580-1150 nm and an optical multichannel analyser. The measurements included DNA stains, fluorescent dyes coupled to antibodies as well as organelle trackers, e.g.

Optimization of Kalpha bursts for photon energies between 1.7 and 7 keV produced by femtosecond-laser-produced plasmas of different scale length.

The conversion efficiency of a 90 fs high-power laser pulse focused onto a solid target into x-ray Kalpha line emission was measured. By using three different elements as target material (Si, Ti, and Co), interesting candidates for fast x-ray diffraction applications were selected. The Kalpha output was measured with toroidally bent crystal monochromators combined with a GaAsP Schottky diode.