Kombucha fermentation and its antimicrobial activity.

Kombucha was prepared in a tea broth (0.5% w/v) supplemented with sucrose (10% w/v) by using a commercially available starter culture. The pH decreased steadily from 5 to 2.

Purification and characterization of beta-1,4-glucosidase from Clostridium papyrosolvens.

Clostridium papyrosolvens CFR-1010 was selected for the anaerobic extracellular production of beta-1,4-glucosidase. The enzyme was purified by alcohol precipitation and DEAE ion-exchange chromatography. Its homogeneity was confirmed by SDS/PAGE.

Genetic and biochemical characterization of novel low molecular weight glutenin subunits in wheat (Triticum aestivum L.).

Two novel low molecular weight subunits of glutenin with relative molecular mass (M(r) values) of 30 and 32 kDa were isolated from the seeds of hexaploid wheat and characterized at genetic and biochemical levels. Among 115 Indian bread wheat cultivars analysed, 40 had a narrow doublet of the new protein bands, 69 had a wide doublet, 3 had only the faster moving band of the doublet, and the remaining 3 cultivars had only the slower moving band. These subunits could be seen in the alkylated glutenin preparations only and the genes for the faster (designated Glu-D4) and slower (designated Glu-D5) moving protein bands of the doublet were located on chromosomes 1D and 7D, respectively.

Destaining of Coomassie Brilliant Blue R-250-stained polyacrylamide gels with sodium chloride solutions.

A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (< 0.