Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D Concentration.

Efficient coordination between Mg and vitamin D maintains adequate Ca levels during lactation. This study explored the possible interaction between Mg (0.3, 0.

SLC41A1 knockout mice display normal magnesium homeostasis.

Transcellular Mg reabsorption in the distal convoluted tubule (DCT) of the kidneys plays an important role in maintaining systemic Mg homeostasis. SLC41A1 is a Na/Mg exchanger that mediates Mg efflux from cells and is hypothesized to facilitate basolateral extrusion of Mg in the DCT. In this study, we generated a SLC41A1 knockout mouse model to examine the role of SLC41A1 in Mg homeostasis.

Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine.

Physiological particularities of the equine heart justify the development of an in vitro model suitable for investigations of the species-specific equine cardiac electrophysiology. Adipose tissue-derived stromal/stem cells (ASCs) could be a promising starting point from which to develop such a cardiomyocyte (CM)-like cell model. Therefore, we compared abdominal, retrobulbar, and subcutaneous adipose tissue as sources for the isolation of ASCs applying two isolation methods: the collagenase digestion and direct explant culture.

Effects of butyrate on ruminal Ca transport: evidence for the involvement of apically expressed TRPV3 and TRPV4 channels.

The ruminal epithelium absorbs large quantities of NH and Ca. A role for TRPV3 has emerged, but data on TRPV4 are lacking. Furthermore, short-chain fatty acids (SCFA) stimulate ruminal Ca and NH uptake in vivo and in vitro, but the pathway is unclear.

Characterization and differentiation potential of mesenchymal stem cells isolated from multiple canine adipose tissue sources.

Background: Mesenchymal stem cells (MSCs) are undifferentiated cells that can give rise to a mesoderm lineage. Adipose-derived MSCs are an easy and accessible source for MSCs isolation, although each source of MSC has its own advantages and disadvantages. Our study identifies a promising source for the isolation and differentiation of canines MSCs.