Estimation of the intramolecular O-H···O═C hydrogen bond energy via the molecular tailoring approach. Part I: aliphatic structures.

A simple and universal method for the estimation of the intramolecular hydrogen bond (HB) energy (E(HB)) in hydroxycarbonyl aliphatic compounds is proposed by the application of the molecular tailoring approach (MTA) based on calculations at the second-order Møller-Plesset MP2 level. The calculation of EHB can be realized by the one optimization and three single point calculations of the energy for each compound with carbonyl and hydroxyl groups involved in HB. The intramolecular hydrogen bond energies estimated for 153 structures (of 102 compounds) ranged from 1.

Genetic and non-genetic determination of repeatability of blood and milk indices in black-and-white cows.

A herd of cows of the same age, kept in a stable system under controlled environmental conditions, was used to estimate the repeatability of diagnostic blood and milk indices in several stages of the I, II and III lactation. Attention was paid to genetic and non-genetic sources of phenotypic variation. Statistically significant values of the regression coefficient were found for the following milk parameters: fat and protein content, milk density, calcium, potassium and lysozyme levels, as well as the following blood parameters: leucocytes' number, lymphocytes and neutrophils number, uric acid content, leucocytes acid phosphatase activity, lysozyme and blood serum alkaline phosphatase activity.

[Polymorphism of acid phosphatase in peripheral blood leukocytes and the hematological and immunological indicators in young bulls of different age].

Studies were carried out on 47 bulls. Determination of polymorphism and the level of analysed indices was performed three times every three months. Statistically significant changes in the level of hematological and immunological indices caused by the growth and development of the examined animals were observed.