Publications by authors named "G Solano"
Recent research suggests that a polygeneric immunogen made from the venoms of the most medically important viperid and elapid snakes in sub-Saharan Africa could elicit a broader antibody response in horses compared to the current EchiTAb-plus-ICP antivenom, especially against neurotoxic elapid venoms. To test this, 25 horses that have been regularly immunized to produce this antivenom were reimmunized with an immunogen containing 22 venoms from various snake species from the genera , , , and both spitting and non-spitting . The plasma collected from these horses was processed using the caprylic acid method to produce an industrial-scale freeze-dried antivenom.
View Article and Find Full Text PDFArticle Synopsis
- - The study evaluated the effectiveness of dynamic body-feed filtration (DBF) in removing bulky solids from equine hyperimmune plasma during snake antivenom production using caprylic acid, comparing various diatomites for optimal filtration performance.
- - C1000 diatomite yielded the best results at 90 g/L concentration, demonstrating a recovery of immunoglobulins of 108 ± 4% in a scale-up to 50 L batches, while meeting quality specifications for the antivenoms.
- - Unlike traditional open filtration systems, DBF offers enhanced microbiological safety due to its closed system design and faster filtration process, making it a cost-effective and compliant alternative for primary clarification in antivenom manufacturing.
Introduction: Antivenom is a lifesaving medicine for treating snakebite envenoming, yet there has been a crisis in antivenom supply for many decades. Despite this, substantial quantities of antivenom stocks expire before use. This study has investigated whether expired antivenoms retain preclinical quality and efficacy, with the rationale that they could be used in emergency situations when in-date antivenom is unavailable.
View Article and Find Full Text PDFArticle Synopsis
- Injectable snake antivenoms are required to meet specific endotoxin content standards for safe clinical use.
- The amebocyte lysate (LAL) test was performed on various commercial antivenoms, revealing some exceeded the acceptable endotoxin limits.
- The findings highlight the importance of incorporating endotoxin testing into regular evaluations by both manufacturers and regulatory bodies.
The present work explores the genotoxicity of the fungicides iprodione (IP) and tebuconazole (TB) using the Allium cepa assay as an in vivo biological model. Both short-term and long-term exposures were studied, revealing concentration- and time-dependent cytological and genotoxic effects. IP exhibited genotoxicity over a wider concentration range (5-50 µg/ml) and required 30 h of exposure, while TB showed genotoxicity at higher concentrations (10 and 30 µg/ml) within a 4-h exposure period.
View Article and Find Full Text PDF