Misregulation of mitochondria-lysosome contact dynamics in Charcot-Marie-Tooth Type 2B disease Rab7 mutant sensory peripheral neurons.

Inter-organelle contact sites between mitochondria and lysosomes mediate the crosstalk and bidirectional regulation of their dynamics in health and disease. However, mitochondria-lysosome contact sites and their misregulation have not been investigated in peripheral sensory neurons. Charcot-Marie-Tooth type 2B disease is an autosomal dominant axonal neuropathy affecting peripheral sensory neurons caused by mutations in the GTPase Rab7.

Optimising curve fitting techniques to look for standardisation of the analysis of defocus curves derived from multifocal intraocular lenses.

Introduction: To establish the most appropriate curve fitting method to allow accurate comparison of defocus curves derived from intraocular lenses (IOLs).

Methods: Defocus curves were plotted in five IOL groups (monofocal, extended depth of focus, refractive bifocal, diffractive bifocal and trifocal). Polynomial curves from 2nd to 11th order and cubic splines were fitted.

Mitochondria-lysosome contact site dynamics and misregulation in neurodegenerative diseases.

Neurons rely heavily on properly regulated mitochondrial and lysosomal homeostasis, with multiple neurodegenerative diseases linked to dysfunction in these two organelles. Interestingly, mitochondria-lysosome membrane contact sites have been identified as a key pathway mediating their crosstalk in neurons. Recent studies have further elucidated the regulation of mitochondria-lysosome contact dynamics via distinct tethering/untethering protein machinery.

One-year post-operative comparison of visual function and patient satisfaction with trifocal and extended depth of focus intraocular lenses.

Purpose: To evaluate visual performance with trifocal and extended depth of focus IOL at 1 year post-operatively.

Setting: BMI Southend Hospital.

Design: Cohort study.

Predicting the Postoperative Addition Power of a Multifocal Intraocular Lens at the Spectacle Plane.

Purpose: To establish a simple clinical method of predicting addition power achieved with a multifocal intraocular lens (IOL).

Methods: In this prospective cohort study, 41 patients were bilaterally implanted with the Bi-Flex MY multifocal IOL (Medicontur) with +3.50 diopters (D) near addition power.