Demonstration of amoeboid and ramified microglial cells in pre- and postnatal bovine brains by lectin histochemistry.

In embryonic, fetal and postnatal bovine brains the development and distribution of microglial cells was examined by lectin histochemistry, using the isolectin B4 from Griffonia simplicifolia (GSA I-B4), the lectin from Ricinus communis (RCA-I), and mistletoe lectin (ML I). With GSA I-B4 and ML I, different types of microglial cells, i.e.

Effects of trypsinization and microwave treatment on lectin labelling of microglial cells in paraffin-embedded sections from pre- and postnatal bovine brains.

The effects of microwave heat treatment on lectin histochemical staining of microglial cells with Griffonia simplicifolia B4 isolectin (GSA I-B4) and Ricinus communis agglutinin-I (RCA-I) in paraffin-embedded pre- and postnatal bovine brain tissue fixed in two different fixatives (Bouin's fluid and 4% neutral buffered formaldehyde) were examined, and the results compared with lectin labelling obtained in untreated and trypsinized serial sections. The results indicate that lectin labelling of bovine microglial cells depends on the kind of lectin applied, the fixative used for tissue preservation, the isotype of microglia to be labelled, and the pretreatment of tissue sections. In brain tissue fixed in Bouin's fluid, GSA I-B4 staining of both microglial isotypes, i.

Lectin labelling of amoeboid and ramified microglial cells in the telencephalon of ovine fetuses with the B4 isolectin from Griffonia simplicifolia.

The topographical distribution of amoeboid and ramified microglial cells in the telencephalon of sheep fetuses at 60-149 days of gestation was examined by lectin histochemistry. Biotinylated B4 isolectin from Griffonia simplicifolia labelled both types of cell in coronal paraffin wax sections fixed in different fixatives. The most intense staining was seen in sections fixed in Bouin's fluid.