Effects of pre-farrowing sow vaccination against on offspring colonisation and lung lesions.

This study investigated colonisation and lung lesions at slaughter in pigs from vaccinated (V) and non-vaccinated (NV) sows, in two herds (A and B). In each herd, two sow batches were V against with a commercial bacterin at six and three weeks before farrowing and two sow batches remained NV. From each sow batch, laryngeal swabs were collected from the litters of five primiparous sows at weaning and seven days post-weaning.

Porcine reproductive and respiratory syndrome virus (PRRSV): a ring test performed in Germany to assess RT-PCR detection methods.

Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine. Due to genetic variation between the European and the US genotype as well as within both genotypes detection of PRRSV is a diagnostic challenge. This paper reports on a ring test to compare different established reverse transcriptase polymerase chain reaction methods applied routinely in 16 different laboratories in Germany.

Antibody prevalence of hog cholera, bovine viral diarrhoea and Aujeszky's disease virus in wild boars in northern Germany.

During the hunting season 1990/1991 a total of 841 blood samples was collected from shot wild boar corresponding to about 2.11% of the total hunting bag in Lower Saxony. All the sera were screened for neutralizing antibodies (nAb) to hog cholera virus (HCV) and bovine viral diarrhoea virus (BVDV) by direct neutralizing peroxidase linked antibody (NPLA) assay.

Clinical, virological and serological findings after intranasal inoculation of pigs with bovine viral diarrhoea virus and subsequent intranasal challenge with hog cholera virus.

Five groups of weaner pigs were intranasally inoculated with constant doses of bovine viral diarrhoea virus (BVDV) strain OSLOSS/2482. Four weeks post primary inoculation (p.p.

[A sensitive enzyme-linked immunosorbent assay (ELISA) for the serological detection of antibodies against the European hog cholera virus].

An ELISA for the detection of antibodies against hog cholera virus (HCV) was developed. The HCV-specific glycoprotein gp53 served as diagnostic antigen after immobilization using a monoclonal capture antibody. Due to the higher affinity of HCV-specific antibodies to the viral gp53, sera cross reacting with bovine viral diarrhea (BVD) virus were discriminated by the slope of the titration curves.