Improving cryo-EM grids for amyloid fibrils using interface-active solutions and spectator proteins.

Preparation of cryoelectron microscopy (cryo-EM) grids for imaging of amyloid fibrils is notoriously challenging. The human islet amyloid polypeptide (hIAPP) serves as a notable example, as the majority of reported structures have relied on the use of nonphysiological pH buffers, N-terminal tags, and seeding. This highlights the need for more efficient, reproducible methodologies that can elucidate amyloid fibril structures formed under diverse conditions.

[Outcome of surgery in 300 cases of hyperthyroidism, including 40 with cardiotoxicity (author's transl)].

Of 300 patients who underwent subtotal thyroidectomy, 204 had Graves' disease, 52 solitary toxic goiter and 39 multinodular toxic goiter. Cardiotoxicity was present in 40 cases. Immediate post-operative complications could be avoided by the use of propranolol, neuroleptanalgesia and, in a few very severe cases, hypothermia.