Publications by authors named "G Sannier"
Article Synopsis
- HIV-1 envelope glycoprotein (Env) structure affects the immune response, with "closed" forms evading certain antibodies while "open" forms are vulnerable to others.
- Infected CD4+ T cells show that downmodulation of CD4 occurs before HIV-1 mRNA expression, and these cells mainly express "closed" Envs, resistant to non-neutralizing antibodies (nnAbs).
- The study challenges the effectiveness of nnAbs in targeting productively infected cells for HIV-1 treatment, as attempts with nnAbs did not reduce viral replication in humanized mouse models.
Article Synopsis
- - The study analyzes plasma samples from 318 COVID-19 patients to understand how RNAemia, delayed antibody responses, and inflammation affect patient outcomes, revealing four distinct patient clusters based on severity and survival probability.
- - Critically ill patients were categorized into good prognosis and high-fatality clusters, while non-critical survivors were divided into high and low early antibody responders, each showing different patterns in antibody development and inflammation.
- - The findings indicate that high-fatality patients have specific genomic signatures linked to severe COVID-19, and both critical and non-critical patients with delayed antibody responses exhibit persistent interferon (IFN) activity, suggesting that high IFN levels might hinder the body's ability to build effective immunity.
Broadly applicable methods to identify and characterize antigen-specific CD4 and CD8 T cells are key to immunology research, including studies of vaccine responses and immunity to infectious diseases. We developed a multiplexed activation-induced marker (AIM) assay that presents several advantages compared to single pairs of AIMs. The simultaneous measurement of four AIMs (CD69, 4-1BB, OX40, and CD40L) creates six AIM pairs that define CD4 T cell populations with partial and variable overlap.
View Article and Find Full Text PDFSpontaneous transcription and translation of HIV can persist during suppressive antiretroviral therapy (ART). The quantity, phenotype, and biological relevance of this spontaneously "active" reservoir remain unclear. Using multiplexed single-cell RNAflow-fluorescence in situ hybridization (FISH), we detect active HIV transcription in 14/18 people with HIV on suppressive ART, with a median of 28/million CD4 T cells.
View Article and Find Full Text PDFCellular immune defects associated with suboptimal responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccination in people receiving hemodialysis (HD) are poorly understood. We longitudinally analyze antibody, B cell, CD4, and CD8 T cell vaccine responses in 27 HD patients and 26 low-risk control individuals (CIs). The first two doses elicit weaker B cell and CD8 T cell responses in HD than in CI, while CD4 T cell responses are quantitatively similar.
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