A handheld biofluorometric system for acetone detection in exhaled breath condensates.

As a marker of human metabolism, acetone is important for lipid metabolism monitoring and early detection of diabetes. In this study, we developed a handheld biosensor for acetone based on fluorescence detection by utilizing the enzymatic reaction of secondary alcohol dehydrogenase (S-ADH) with β-nicotinamide adenine dinucleotide (NADH, = 340 nm, = 490 nm). In the reaction, NADH is oxidized when acetone is reduced to 2-propanol by S-ADH, and the acetone concentration can be measured by detecting the amount of NADH consumed in this reaction.

MRI guided copper deprivator activated immune responses and suppressed angiogenesis for enhanced antitumor immunotherapy.

Copper plays an important role in the regulation of PD-L1, suggesting that reducing copper levels within tumors may enhance anti-cancer immunotherapy. Tumor microenvironment responsive copper nanodeprivator (TMECN) was developed for enhancing immunotherapy of tumor via the cross-link of mercaptopolyglycol bipyridine and dimercaptosuccinic acid modifying FePt nanoalloy using the disulfide bond. Upon entering tumor cells, the disulfide bond in TMECN is cleaved by the overexpressed glutathione, exposing abundance of sulfhydryl groups.

The synthesis of the novel toxin-colibactin and its mechanisms of tumorigenesis of colorectal cancer.

is part of the normal flora of the human gut and performs vital functions; however, certain strains can cause disease in the host, impairing gut function and adversely affecting overall health. The pks gene cluster in the B2 serogroup encodes colibactin, a secondary metabolite and a potential gut toxin. However, the mechanism underlying colibactin production in is complex, and the function of the pks gene cluster is not fully understood.

HPClas: A data-driven approach for identifying halophilic proteins based on catBoost.

Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean-up, and energy production. Generally, halophilic proteins are discovered and characterized through labor-intensive and time-consuming wet lab experiments.

Genome-wide identification and expression analysis of the gene family in pea.

Peas ( L.) serve as a vital model for plant development and stress research. The () gene family, encoding essential motor proteins, remains understudied in peas.