Publications by authors named "G S Fragiadakis"
Human immune systems are highly variable, with most variation attributable to non-genetic sources. The gut microbiome crucially shapes the immune system; however, its relationship with the baseline immune states of healthy humans remains incompletely understood. Therefore, we performed multi-omic profiling of 110 healthy participants through the ImmunoMicrobiome study.
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- - This study focused on developing a new imaging-based method called single-cell spatial transcriptomics (iSCST) to analyze archived, formalin-fixed, paraffin-embedded (FFPE) tissue samples from inflammatory bowel disease (IBD) patients, which helps to preserve the spatial context of various cell types within the tissue.
- - Researchers benchmarked three iSCST platforms by analyzing 57 FFPE mucosal biopsies from IBD patients and healthy controls, allowing for detailed cell analysis and gene expression profiling through a consistent data processing pipeline.
- - A custom gene panel showed the best performance for detecting and quantifying different cell subsets, revealing significant findings related to inflammation and treatment responses in ulcerative colitis, which could enhance
Objective: To investigate if a positive result on ReceptivaDx for evaluation of B-cell lymphoma 6 (BCL6), a proposed marker of progesterone resistance associated with impaired uterine receptivity, correlates with a suboptimal profile of receptivity-associated markers in the window of implantation using the endometrial receptivity array and single-nucleus transcriptomic analysis.
Design: Retrospective clinical cohort study; pilot study of single-nucleus RNA sequencing of prospectively collected window of implantation endometrium undergoing ReceptivaDx BCL6 evaluation.
Setting: Academic center.
Article Synopsis
- The study investigates the connection between interferon and systemic lupus erythematosus (SLE), focusing on how transposable elements (TEs) might influence SLE symptoms, particularly autoantibody production.
- Researchers analyzed RNA-sequencing data from immune cells in 120 SLE patients, identifying over 27,000 TEs and observing 731 that were differentially expressed among various SLE phenotypes.
- Results indicate that specific TEs are linked to genes responsible for antiviral responses and IFN signaling, highlighting their potential role in understanding and treating SLE.
Article Synopsis
- This study focused on managing rheumatoid arthritis (RA) by analyzing blood cells from patients and healthy individuals to identify specific cell types and their roles in disease activity.
- Researchers discovered 18 distinct types of immune cells, noting that patients with more severe RA had an increase in certain T cells, while those in remission showed fewer nonclassical monocytes.
- The study also highlighted key gene expressions related to inflammation and disease, providing insights into the complex biological processes that contribute to RA's variability in severity.