Comparative Genomic Analysis of Plasmids Identified in Food Isolates.

is one of the leading bacterial causes of gastroenteritis worldwide. It frequently contaminates poultry and other raw meat products, which are the primary sources of infections in humans. Plasmids, known as important mobile genetic elements, often carry genes for antibiotic resistance, virulence, and self-mobilization.

Rubisco packaging and stoichiometric composition of the native β-carboxysome in Synechococcus elongatus PCC7942.

Carboxysomes are anabolic bacterial microcompartments that play an essential role in CO2 fixation in cyanobacteria. This self-assembling proteinaceous organelle uses a polyhedral shell constructed by hundreds of shell protein paralogs to encapsulate the key CO2-fixing enzymes Rubisco and carbonic anhydrase. Deciphering the precise arrangement and structural organization of Rubisco enzymes within carboxysomes is crucial for understanding carboxysome formation and overall functionality.

Engineering CO-fixing modules in E. coli via efficient assembly of cyanobacterial Rubisco and carboxysomes.

Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) is the central enzyme for converting atmospheric CO into organic molecules, playing a crucial role in the global carbon cycle. In cyanobacteria and some chemoautotrophs, Rubisco complexes, along with carbonic anhydrase, are enclosed within specific proteinaceous microcompartments, known as carboxysomes. The polyhedral carboxysome shell ensures a dense packaging of Rubisco and creates a high-CO internal environment to facilitate the fixation of CO.

Rubisco packaging and stoichiometric composition of a native β-carboxysome.

Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria. This self-assembling proteinaceous organelle encapsulates the key CO-fixing enzymes, Rubisco and carbonic anhydrase, using a polyhedral shell constructed by hundreds of shell protein paralogs. Deciphering the precise arrangement and structural organization of Rubisco enzymes within carboxysomes is crucial for understanding the formation process and overall functionality of carboxysomes.