Combination of high-fat/high-fructose diet and low-dose streptozotocin to model long-term type-2 diabetes complications.

The epidemic of type 2 diabetes mellitus (T2DM) is fueled by added fructose consumption. Here, we thus combined high-fat/high-fructose diet, with multiple low-dose injections of streptozotocin (HF/HF/Stz) to emulate the long-term complications of T2DM. HF/HF/Stz rats, monitored over 56 weeks, exhibited metabolic dysfunctions associated with the different stages of the T2DM disease progression in humans: an early prediabetic phase characterized by an hyperinsulinemic period with modest dysglycemia, followed by a late stage of T2DM with frank hyperglycemia, normalization of insulinemia, marked dyslipidemia, hepatic fibrosis and pancreatic β-cell failure.

[Morgagni's hernia: Report of a case treated by videolaparoscopy].

Morgagni's hernia is a rare congenital disorder, usually asymptomatic, but may cause respiratory or gastrointestinal symptoms. We reviewed the clinical findings of a patient with a Morgagni's hernia, diagnosed and treated in our department. The Morgagni's hernia caused various symptoms in our patient and surgery was performed via laparoscopy.

Regional and subcellular distribution of the receptor-targeting protein PIST in the rat central nervous system.

Protein interacting specifically with Tc10, PIST, is a Golgi-associated sorting protein involved in regulating cell-surface targeting of plasma membrane receptors. The present study provides the first comprehensive description of PIST distribution in the mammalian central nervous system and of its subcellular localization by immunocytochemistry. PIST is distributed widely throughout the neuraxis, predominantly associated with neuronal cell bodies and dendrites.

Altered morphine-induced analgesia in neurotensin type 1 receptor null mice.

Both neurotensin (NT) and opioid agonists have been shown to induce antinociception in rodents after central administration. Besides, previous studies have revealed the existence of functional interactions between NT and opioid systems in the regulation of pain processing. We recently demonstrated that NTS1 receptors play a key role in the mediation of the analgesic effects of NT in long-lasting pain.