Publications by authors named "G R Sharmila"

Introduction: Thermotolerant microbes are a group of microorganisms that survive in elevated temperatures. The thermotolerant microbes, which are found in geothermal heat zones, grow at temperatures of or above 45°C. The proteins present in such microbes are optimally active at these elevated temperatures.

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In recent years, plant based scaffold due to its inherent properties such as mechanical stability, renewability, easy mass production, inexpensiveness, biocompatibility and biodegradability with low toxic effects have received much attention in the field of bone tissue engineering. Design of good tissue compatible plant based polymer scaffold plays a vital role in biomedicine, nanomedicine and in various tissue engineering applications. The present study focused on the fabrication of a novel herbal scaffold using the medicinal plants Spinacia oleracea (SO) and Cissus quadrangularis (CQ) extracts incorporated with Alginate (Alg), Carboxy Methyl Cellulose (CMC) by lyophilization method.

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The sequential optimization of carboxymethylation of pectin by Plackett-Burman (PB) design and response surface methodology (RSM) was reported in this study. PB design was employed to screen the six process variables (ethanol concentration, liquid-polymer ratio, NaOH concentration, CAA concentration, temperature and time). Central composite design (CCD) was used to study the interaction effects of ethanol concentration, NaOH concentration, CAA concentration and time on degree of substitution (DS) in carboxymethylated pectin (CMP).

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Herein, we report a novel, disposable electrochemical sensor for the detection of nitrite ions in food samples based on the sonochemical synthesized orthorhombic CaFeO (CFO) clusters modified screen printed electrode. As synthesized CFO clusters were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transformer infrared spectroscopy (FT-IR), Thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and amperometry (i-t). Under optimal condition, the CFO modified electrode displayed a rapid current response to nitrite, a linear response range from 0.

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Article Synopsis
  • Cereals and pulses are vital for their protein content, but anti-nutritional factors like Kunitz trypsin inhibitors (KTI) hinder nutrient absorption and cause metabolic disorders.
  • A KTI degrading protein (BPC) was discovered and characterized from Bacillus subtilis CFR5, exhibiting significant similarities to another enzyme and effectively cleaving KTI to produce inactive fragments.
  • BPC shows optimal activity at 40°C and pH 8.0, is enhanced by calcium, and mutational studies identify critical residues that influence its catalytic function, revealing potential for improving the nutritional value of these food sources.
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