Publications by authors named "G R Mills"
Multiplexed tissue imaging (MTI) technologies enable high-dimensional spatial analysis of tumor microenvironments but face challenges with technical variability in staining intensities. Existing normalization methods, including z-score, ComBat, and MxNorm, often fail to account for the heterogeneous, right-skewed expression patterns of MTI data, compromising signal alignment and downstream analyses. We present UniFORM, a non-parametric, Python-based pipeline for normalizing both feature- and pixel-level MTI data.
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- A study evaluated the effectiveness of invasive versus conservative treatment strategies in older women with non-ST-elevation acute coronary syndrome (NSTEACS), addressing the lack of representation of this group in clinical trials.
- The analysis included 717 women and found that the invasive strategy showed a lower risk of myocardial infarction (MI) and urgent revascularization compared to the conservative approach, although it didn't significantly reduce overall mortality rates.
- The findings suggest that while invasive treatment is beneficial for reducing MI in older women with NSTEACS, it does not impact the overall risk of death or other severe outcomes after one year.
Therapeutic strategies targeting the DNA damage response, such as poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi), have revolutionized cancer treatment in tumors deficient in homologous recombination (HR). However, overcoming innate and acquired resistance to PARPi remains a significant challenge. Here, we employ a genome-wide CRISPR knockout screen and discover that the depletion of ubiquitin-activating enzyme E1 (UBA1) enhances sensitivity to PARPi in HR-proficient ovarian cancer cells.
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