Publications by authors named "G R Bedford"
Article Synopsis
- Researchers developed engineered enzymes that can add acylation (a chemical modification) to certain proteins, helping to explore how these changes affect gene regulation and epigenetics.
- They discovered that targeting one of these engineered enzymes to gene promoters boosts their activation, but targeting it to enhancers is less effective.
- The team made a mutation in a human enzyme that reduces toxicity while keeping its functions, which improves methods for delivering therapies—this work aids in understanding and editing the epigenome for research and potential medical advancements.
Persistent tailoring of MSC activation through genetic priming.
Mol Ther Methods Clin Dev
September 2024
Mesenchymal stem/stromal cells (MSCs) are an attractive platform for cell therapy due to their safety profile and unique ability to secrete broad arrays of immunomodulatory and regenerative molecules. Yet, MSCs are well known to require preconditioning or priming to boost their therapeutic efficacy. Current priming methods offer limited control over MSC activation, yield transient effects, and often induce the expression of pro-inflammatory effectors that can potentiate immunogenicity.
View Article and Find Full Text PDFMesenchymal stem/stromal cells (MSCs) are an attractive platform for cell therapy due to their safety profile and unique ability to secrete broad arrays of immunomodulatory and regenerative molecules. Yet, MSCs are well known to require preconditioning or priming to boost their therapeutic efficacy. Current priming methods offer limited control over MSC activation, yield transient effects, and often induce expression of pro-inflammatory effectors that can potentiate immunogenicity.
View Article and Find Full Text PDFEngineered transactivation domains (TADs) combined with programmable DNA binding platforms have revolutionized synthetic transcriptional control. Despite recent progress in programmable CRISPR-Cas-based transactivation (CRISPRa) technologies, the TADs used in these systems often contain poorly tolerated elements and/or are prohibitively large for many applications. Here, we defined and optimized minimal TADs built from human mechanosensitive transcription factors.
View Article and Find Full Text PDFIntraoperative Vasopressor Usage in Free Tissue Transfer: Should We Be Worried?
J Reconstr Microsurg
January 2022
Article Synopsis
- The debate over vasopressor use in microsurgery has been ongoing, with traditional views advocating against their use due to concerns about poor perfusion and complications during free tissue transfer surgery.
- A retrospective review of 777 cases at a high-volume center showed that 59.1% of patients received vasopressors without an increased flap failure rate, but there was a noted increase in microvascular thrombosis.
- The study concludes that continuous administration of vasopressors could be safer compared to intermittent bolus doses, supporting the idea that their use in surgery may not be as harmful as previously thought.