Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice.

Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential.

Poly(epsilon-caprolactone) biomaterial sterilized by E-beam irradiation.

The effects of ionizing radiation (electron beam) on poly(epsilon-caprolactone) (PCL) were studied by analyzing changes in viscosity-average and weight-average molecular weight and radius of gyration, and by performing sol-gel analysis and swelling tests. Samples were irradiated under various conditions: solid and molten PCL in the presence or absence of air. The overall efficiency of crosslinking is higher for samples irradiated in the molten state than in the solid state, and is reduced in the presence of oxygen.

The effect of gamma-radiation on nitroimidazole derivatives.

The effect of gamma irradiation, in doses from 10 to 50 kGy on physical and chemical properties of metronidazole, ornidazole and tynidazole in solid state has been studied. Results of the measurements by the UV, IR, TLC, EPR, DSC methods revealed the presence of free radicals, products of decomposition, an increase in the melting point and a decrease or increase in the content of the substance studied. Microbiological assays proved that sterilization of the compounds studied was effective even with the smallest irradiation dose applied.