A computer algorithm to determine the recognition site of restriction enzymes.

A new algorithm is proposed to determine the type-II restriction endonucleases' recognition site knowing the digested DNA sequence and fragment lengths in an actual case. The algorithm is implemented for the Commodore 64 microcomputer.

PMAP, PMAPS: DNA physical map constructing programs.

Computer programs are described, which facilitate the construction of the restriction site (physical) map of DNA molecules. By knowing the length of each fragment and its degree of error in the single and the double restriction enzyme digestions, the programs give all the possibilities for the physical map. This method is applicable to linear DNA molecules.

The detailed physical map of the temperate phage 16-3 of Rhizobium meliloti 41.

Restriction cleavage maps for enzymes EcoRI, BamHI, PstI, PvuII, XbaI and EcoRV of Rhizobium meliloti temperate phage 16-3 have been established. Together with the earlier maps (HindIII, KpnI, HpaI, BglII) 98 restriction sites, 'evenly' distributed, have been mapped along the phage genome, including the so far unmarked silent region of the chromosome. All the restriction maps have been fitted to each other by computer optimalization.