Complete sequence of ovine alpha s2-casein messenger RNA.

The primary structure of mRNA coding for ovine alpha s2 casein has been determined by chemical sequencing of three cDNA clones and the primer extension products of the longest one. The mRNA was 1,024 nucleotides long, excluding the poly(A) tail. The length of the 5' noncoding, coding and 3' noncoding regions was 53, 669 and 302 nucleotides, respectively.

[Control of the initiation step in eukaryotic cells].

This article presents some arguments in favor of post-transcriptional regulation of protein synthesis. The macromolecules inducing initiation have been described. The control mechanisms of initiation have been studied in reticulocytes and cells treated with interferon.

Purification and properties of the major tRNAHis from sheep liver.

The major isoacceptor of tRNAHis from sheep liver was purified. Two different methods were described which both took advantage of the presence of the hypermodified Q nucleotide in this tRNA. In the first procedure, CNBr treatment of tRNA which was previously enriched in tRNAHis greatly increased the efficiency of the subsequent chromatographic steps.