Publications by authors named "G Pauli"
To date, quantitative analysis of proanthocyanidin (PAC) containing materials including plant extracts and fractions depends on colorimetric assays or phloroglucinolysis/thiolysis combined with UV-HPLC analysis. Such assays are of limited accuracy, particularly lack specificity, require extensive sample preparation and degradation, and need appropriate physical reference standards. To address this analytical challenge and toward our broader goal of developing new plant-sourced biomaterials that chemically and mechanically modulate the properties of dental tissue for clinical interventions, we have characterized 12 different PAC DESIGNER (Depletion and Enrichment of Select Ingredients Generating Normalized Extract Resources) materials.
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- * The study analyzes a comprehensive database called NAPRALERT, which includes over 38,000 experiments, helping to identify NPs that have shown promise in pain relief and related biological effects.
- * The report provides tools for users to effectively navigate the vast data set and emphasizes the importance of combining traditional medicinal uses with scientific evidence for selecting potential NP leads in pain research.
This exploratory study was designed to identify factors implicating microbial influence on medicinal plant metabolomes. Utilizing a whole-microbiome approach, amplicon sequencing was used to identify the makeup of fungal and bacterial assemblages from endophytic (interior) and epiphytic (external) environments in two different sets of congeneric host-plant pairs, with collection of multiple samples of two medicinal plant species () and two generic analogs (). Diversity analysis of microbial assemblages revealed the influence of three primary factors driving variance in microbial community composition: host-plant taxonomy, the compartmentalization of microbial communities within discrete plant parts, and the scale of distance (microhabitat heterogeneity) between sampling locations.
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- A new NMR-based method for identifying and quantifying amino acids was developed, utilizing both high-field (600 MHz) and benchtop (60 MHz) NMR instruments to analyze four amino acids and their salts.
- The methodology demonstrated that key NMR parameters, like chemical shifts, remained consistent regardless of the magnetic field strength, while introducing an alternative identification technique using C NMR patterns from a specific type of NMR measurement (ed-HSQC) for clearer documentation.
- The quantification of amino acid reference materials using a qNMR approach showed that the samples analyzed were generally of high purity, confirming the effectiveness of the developed techniques.
Objective: Investigate the bioactivity and stability of Rhodiola rosea (RR) fractions as a natural source of prodelphinidin gallate (PDg) on dentin collagen via analysis of the viscoelastic and resin-dentin adhesive properties of the dentin matrix.
Methods: The biomimicry and stability of RR subfractions (F1, F2, F3 and F4) with collagen were determined by dynamic mechanical analysis (DMA). DMA used a strain sweep method to assess the dentin matrix viscoelastic properties [storage (E'), loss (E"), and complex (E*) moduli and tan δ] after treatment, 7-, 30- and 90-days of storage in simulated body fluids (SBF).