Publications by authors named "G Pallocca"
Cell-based test methods with a phenotypic readout are frequently used for toxicity screening. However, guidance on how to validate the hits and how to integrate this information with other data for purposes of risk assessment is missing. We present here such a procedure and exemplify it with a case study on neural crest cell (NCC)-based developmental toxicity of picoxystrobin.
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- In Europe, rodent studies are the main method used to assess neurotoxicity, but they are expensive and raise ethical concerns, leading many to seek alternatives.
- There is a growing public demand for safer chemicals, as many on the market haven't been thoroughly tested for neurotoxic effects, prompting research into New Approach Methods (NAMs) to replace animal testing.
- The European Partnership for the Assessment of Risks from Chemicals (PARC) is working on NAMs to evaluate neurotoxicity, aiming to create faster and cheaper testing methods that can help regulatory agencies and industries improve safety assessments.
Every test procedure, scientific and non-scientific, has inherent uncertainties, even when performed according to a standard operating procedure (SOP). In addition, it is prone to errors, defects, and mistakes introduced by operators, laboratory equipment, or materials used. Adherence to an SOP and comprehensive validation of the test method cannot guarantee that each test run produces data within the acceptable range of variability and with the precision and accuracy determined during the method validation.
View Article and Find Full Text PDFTo transfer toxicological findings from model systems, e.g. animals, to humans, standardized safety factors are applied to account for intra-species and inter-species variabilities.
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- The establishment of initiatives centered on the Three Rs (Replacement, Reduction, and Refinement) has been bolstered by the adoption of new protective regulations for animals in scientific research.
- Recent articles have been published to review the growth and current activities of European Three Rs centres and platforms, detailing their financial structures, core functions, and ongoing projects.
- These centres serve as crucial hubs for promoting the Three Rs principles, offering models for sustainability and facilitating information sharing in their countries.