Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16.

Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular and humoral chains of immune system, and to analyze possible mechanisms of its antimetastatic action.

Materials And Methods: XCV was administered triply with 3-day intervals after surgical removal of experimental melanoma В-16 in C57Bl/6 mice. Cytotoxic activity (CTA) of splenocytes against target cells К-562 as well as CTA of splenocytes, peritoneal macrophages (PM) and blood serum against melanoma В-16 target cells were determined using МТТ test.

[Corrective action of bacillary probiotic on the immune system of animals in experimential dysbacteriosis].

Is established that at intestinal dysbacteriosis in the organism of experimental animals develop reactions of inflammatory type (early and late), leading to an accumulation in the blood serum of humoral immunity factors, blocking reactions mediated by lymphocytes ormacrophages, while maintaining their cytotoxic activity. Introduction of animals of tne probiotic endosporin promotes restoration of quantitative and qualitative indicators of intestinal microbiocenosis and the normalization of the immune system.

[Effect of dendritic cell autovaccine on the treatment outcome in patients with ovarian cancer].

During I/II phase clinical trial in ovarian cancer (OC) patients two types of autologous anticancer vaccines based on dendritic cells have been tested, and a comparative analysis of their effectiveness have been performed. It was shown that the anticancer vaccines based on DC, "loaded" with autologous tumor cell lysate obtained by treatment of tumor cells by cytotoxic lectins B. subtilis had higher efficiency, compared with the standard DC--autovaccine.

[Lectin activity of antitumor substances synthesized by Bacillus subtilis B-7025].

Nutrient medium, which is optimal for synthesis of biologically active substances and allows obtaining stable outflow of Bacillus subtilis B-7025 to the culture medium, has been selected under the conditions of the bacillus periodic cultivation. It has been established that these substances are extracellular lectins with high activity and carbohydrate specificity to fructose-1.6-di phosphate, N-acetylneuraminic acid and glucose derivatives: D-glucuronic acid and D-glucosamine.

[The efficacy of using low doses of immunomodulators for treating experimental tumors].

The data about the ability of various substances to cause definite reactions in biological objects are adduced, and, in particular, about the efficacy of a small doses of antitumoral chemopreparations in experiment. The results of investigation of the application efficacy of various doses of immunomodulating agents, such as arbisol, nucleoplat, the substrate from gram-negative bacteria Yersinia enterocolitica, antitumoral vaccine from the tumoral cells are presented for the cancer chemotherapy. While application of super-small doses of an antitumoral vaccine there was noted statistically significant inhibition of tumoral growth.