[Cytostatic effects of bifolar and chlofiden. Effect on protein synthesis and cell cycle].

Study of influence of new antitumor preparations bifolar and chlofiden on rat liver protein biosynthesis in vivo and on sarcoma-45 in vitro was carried out using labeled precursor 3H-leucine. It was shown that the preparations inhibited protein synthesis in normal and malignant tissues. Mitotic cycle and its phases were investigated with 3H-tymedin.

[Protein synthesizing cell apparatus in malignant growth and pharmacotherapy by chlofiden].

Data on the influence of a new antitumor preparation chlofiden on the general contents of rat liver ribosomes and sarcoma 45 and their division on free and membrane of membrane bound and decrease of free ribosomes during tumor growth supposed synthesis of specific proteins bound are given in the paper. It was shown that in the liver of tumor bearing rats total and membrane bound ribosomes decreased and the level of free ribosomes increased. High contents of free ribosomes in sarcoma 45 may testify increase of intracellular protein synthesis including processes of cell growth and division as well as the tendency for increase.

[Mechanism of action of a novel antitumor preparation of chlofiden. Effect on RNA synthesis].

Study of the influence of the antitumor preparation chlofiden on RNA synthesis by sarcoma 45 and liver cells was carried out using 14C-orotic acid. Chlofiden was shown to activate RNA transport from the nucleus to the cytoplasm, and to reduce label incorporation into the nuclear and cytoplasmic RNA. Chlofiden increased the rate of RNA processes, which promoted some increase of label incorporation into low molecular RNA fraction of the nucleus and the cytoplasm.

[Effect of bifolar and chlofiden on DNA synthesis of sarcoma 45 cells and on mitotic activity of rat small intestine epithelium].

Results of the study on the influence of new antitumor preparations chlofiden and bifolar on sarcoma 45 DNA synthesis and on mitotic activity are given. It was shown that bifolar and chlofiden led to inhibition of label incorporation into DNA (3H-thymidine). Inhibition of mitotic activity after injection of both preparations in toxic doses (DL50) was revealed.

[Cytotoxic effect of antineoplastic substances and their effect on DNA synthesis and cGMP level in tumor and normal tissues].

The cytostatic effect of antitumour agents line on the level of malignantly changed actively proliferating system (tumour growth) and normal actively proliferating system (splin) was investigated on the basis of DNA synthesis and cGMP rate. Studies of these antitumour agents were carried out on three tumour growth models (Pliss lymphosarcoma, Yablonovskaya glyoblastoma and human glyoblastoma heterografts (subcapsular test). It has been demonstrated that chlofiden, brotheophine, vincristine, adriablastine, natulan, phthorafur have a marked and stable effect of DNA synthesis inhibition, resulting in their marked cytostatic effect and antitumour action stability under the therapy conditions.