Two alkaline RNases and three forms of the enzyme are detected in the cell cytoplasm of the pigling kidney culture transplanted line (IB-RS-2) by gel filtration through Sephadex G-100, G-150 and by chromatography on molselect SE-50. The former two RNases are in complex with the enzyme inhibitor and the latter three forms manifest their action in the pH acid region.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
December 1977
The enzymatic preparations of the RNA-ases from the cells of a continuous line of the sucking pig kidney culture, the blood serum of chicks, and the blood serum of rabbits were isolated and purified. The antiviral action of these enzymes on the homologous culture, chick embryos in infection with the RNA-genom virus of the Newcastle's disease and on rabbits infected with the DNA-genom virus of the Aujeszky's disease was demonstrated. A supposed mechanism of the protective action of "own" RNA-ases is discussed in this work.
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