Squaric esters as peptide stapling reagents.

We report that squaric esters can serve as bifunctional reagents for selective peptide stapling reactions. Formation of the squaric amide staple occurs under mild conditions with amine-containing side chains. We show that short resin-bound peptides are readily stapled on solid phase and that stapling can occur at various relative positions along the peptide and with various amine tether lengths (e.

Effect of rotating monensin plus tylosin and lasalocid on performance, ruminal fermentation, and site and extent of digestion in feedlot cattle.

Two trials were conducted to evaluate the effects of ionophore rotation programs on performance and digestion by feedlot cattle. A 90% concentrate diet was fed with treatments of no ionophore (C), 33 mg lasalocid/kg diet daily (L), 29 mg monensin plus 11 mg tylosin/kg diet daily (MT), and daily (D) and weekly (W) rotation of L and MT. In Trial 1, feedlot performance of 200 crossbred steers (average initial BW 296 kg) was evaluated during a 133-d period.

Nutrition and management of stressed beef calves. An update.

In an earlier report in the Veterinary Clinics: Large Animal Practice, the author discussed some basic principles upon which receiving programs for stressed calves should be based. Additional information has been developed in some of the areas covered in that report and in certain other areas. It is the purpose of this article to discuss some of this new information, after a brief review of the basics.

Effects of receiving and growing diets on compensatory gains of stressed calves.

Three hundred seventy-seven male stocker calves were used to study the effects of three receiving diets, two growing-finishing diets and two levels of shipping shrink on compensatory gains during the post-receiving feeding period. Calves received on a 75% concentrate diet gained 19 kg more during the first 42 d than those received on hay plus .91 kg daily of protein supplement (P less than .

Influence of steam-flaked, steamed-whole or whole shelled corn on performance and digestion in beef steers.

Three trials were conducted to evaluate finishing diets containing 67% steam-flaked corn (SFC), steamed-whole corn (SWC) or whole corn (WC). In a feeding trial, steers fed SWC consumed more (P less than .05) dry matter per day (7.