[Isolation and purification of human blood plasma proteins able to form potassium channels in artificial bilayer lipid membrane].

Protein fraction able to induce K(+)-selective transport across bilayer lipid membrane was isolated from human blood plasma with the use of the detergent and proteolytic enzyme-free method developed at our laboratory. After addition of the studied sample to the artificial membrane in the presence of 100 mM KCl, a discrete current change was observed. No channel activity was recorded in the presence of calcium and sodium ions.

[Characterization of changes in the activity of K+-n-nitrophenylphosphatase of erythrocyte ghosts under the influence of ouabain].

Variation of K+-p-NPP-ase of human ghosts under the action of ouabain (10(-11)-10(-3) M) has been studied. In human ghosts the activity of ouabain-sensitive K+-p-NPP-ase has been found to make up 65% of the total enzyme activity. The activity of ouabain-sensitive K+-p-NPP-ase reaches a maximum level at pH 7.

[Na,K-ATPase activity of erythrocyte ghosts of mammals in the presence of alamethicin and p-nitrophenylphosphate].

After preincubation of erythrocyte ghosts isolated from Sprague-Dawley rats activity of Na, K-ATPase was more successfully manifested, being elevated about 2.5-fold, while the ratio Mg-ATPase/Na, K-ATPase was decreased 3-fold. Optimal ratio of alamethicin/protein was equal to 0.

[Role of peroxides in changes in brain mitochondria energy metabolism following exposure to schizophrenic patients' serum].

Antioxidants normalize some of the characteristics of energy metabolism in mitochondria from the rat cerebral cortex, altered as a result of exposure to blood serum from patients suffering from continuous schizophrenia. Meanwhile, antioxidants proved little effective after administration of blood serum from patients with attach-like schizophrenia. The whole blood serum and low-molecular fractions (ultrafiltrate, protein-free) obtained from it (the first group of patients) increased the intensity of malonic dialdehyde accumulation by the test system, whereas in the second group of patients, this process, exposed to the analogous blood fractions, proceeded less violently.

[Changes in the metabolism of cerebral mitochondria under the influence of sera from schizophrenic patients and their relatives].

With the aid of a polarographic method it was demonstrated that the blood serum of schizophrenic patients and their relatives, unlike the blood serum taken from normal donors evoke a significant change of the metabolism in the mitochondria of the hemispheric cortex. The intensity of oxidation and respiration control under the influence of these sera change similarly, while the intensity of phosphorylation and oxidation with phosphorylation coupling change significantly to a higher degree under the influence of the blood of probands. Changes in the metabolism indices of mitochondria under the influence of these sera to a significant extent are genetically determined.