J Photochem Photobiol B
April 1992
Several parameters of chlorin e6 and its derivative chlorin e6 ethylenediamide have been investigated as these compound are potential sensitizers for photodynamic therapy. A study carried out to compare the cellular uptake of the pigments indicates that chlorin e6 ethylenediamide possesses an enhanced affinity for tumour cells and cellular membranes. Comparison of the uptake in induced sarcoma shows that chlorin e6 ethylenediamide is a much better tumour localizer than chlorin e6.
View Article and Find Full Text PDFThe relationships between the rate of post-irradiated photohaemolysis sensitized by chlorin e6 and parameters such as the light fluence (time of irradiation) and sensitizer concentration were studied. On the basis of the single-parametric approach proposed by Valenzeno and Pooler, it was found that the haemolytic rate varies with the square of both the light fluence and the sensitizer concentration. Thus it can be concluded that, in a single erythrocyte lesion, two chlorin e6 molecules participate, each absorbing one photon.
View Article and Find Full Text PDFLevels of copro- and protoporphyrin in red blood cells obtained from patients with tumors of the stomach, large bowel and thyroid are discussed. The levels of both porphyrins proved markedly increased in patients with gastric and intestinal cancer but were nearly normal in cases of benign tumor of various sites and thyroid cancer.
View Article and Find Full Text PDFData on porphyrin metabolism disturbances under the action of ionizing radiation on the human body are presented. The studies are based on estimating the quantitative content of coproporphyrin in the diurnal urine diuresis of persons irradiated as a result of the Chernobyl accident. The results of examining the population of a number of the zones of close control in Mogilev and Gomel Provinces are considered.
View Article and Find Full Text PDFOn the basis of spectral fluorescence and polarization measurements and results obtained on the luminescence quenching of the membrane fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) by incorporated chlorin e6 (chl e6) molecules, it is shown that the interaction of the water-soluble pigment with smaller unilamellar lipid vesicles occurs by a mechanism of partition between the aqueous and lipid phases (partition coefficient Kp = 6.7 x 10(3) and provides rigid fixing of chl e6 monomers at the boundary between the polar and non-polar parts of the lipid membrane. In terms of inductive resonance electronic excitation energy transfer between DPH and chl e6 (R0 = 36.
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