Publications by authors named "G O Kamoshida"
Unlabelled: imaging of bacterial infection models enables noninvasive and temporal analysis of individuals, enhancing our understanding of infectious disease pathogenesis. Conventional imaging methods for bacterial infection models involve the insertion of the bacterial luciferase LuxCDABE into the bacterial genome, followed by imaging using an expensive ultrasensitive charge-coupled device (CCD) camera. However, issues such as limited light penetration into the body and lack of versatility have been encountered.
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- Endotoxins (LPS) are problematic in bacterial protein production, but researchers developed a method using a special bacterium that lacks LPS.
- They successfully created endotoxin-free proteins, including functional green fluorescent protein and the cytokine TNF-α, and significantly reduced contamination levels.
- This new system also enabled the production of a specific antibody targeting the coronavirus spike protein and a rheumatoid arthritis drug, showcasing its potential for future applications in biotechnology.
Article Synopsis
- The study focuses on understanding the function of the PmrAB two-component system (TCS) in resistance to colistin, a last-resort antibiotic, in extensively drug-resistant (XDR) Gram-negative bacteria.
- Researchers conducted transcriptome analysis to identify the regulatory genes controlled by PmrAB, revealing its responsiveness to environmental factors like pH and metal ions (Fe, Zn, Al).
- The findings highlight PmrAB's role in both environmental adaptation and the development of antibiotic resistance by modifying lipooligosaccharide (LOS) to mitigate toxicity and enhance resistance to colistin and polymyxin B.
Objectives: The third-generation cephalosporin (3GC)-resistant strains have been detected worldwide in humans and animals. Hence, in this study, we evaluated the prevalence and genetic characteristics of 3GC-resistant in livestock, farmers, and patients to further analyse if livestock serves as a potential reservoir of antimicrobial-resistant bacteria.
Methods: Faecal samples were collected from 330 healthy livestock (216 cattle and 114 swine), 61 healthy livestock farmers (52 cattle farmers and 9 swine farmers), and 68 non-duplicate 3GC-resistant isolates were also obtained from the clinical specimens of patients in Japan between 2013 and 2015.
Article Synopsis
- * This study examined how colistin-resistant A. baumannii strains arise, showing that while colistin alone leads to LPS-deficient strains, combining it with other antibiotics results in strains with modified LPS.
- * The findings indicate that LPS-deficient strains are less fit and more susceptible to other treatments, suggesting that LPS-modified strains are more prevalent in clinical settings, highlighting the urgent need to address colistin resistance in healthcare.