Publications by authors named "G N Goud"

Article Synopsis
  • The study investigates how the cellular translation system maintains homochirality (preference for L-amino acids) in proteins, emphasizing the role of enzymes like alanyl-tRNA synthetase (AlaRS).
  • It challenges previous models by demonstrating that AlaRS does not activate D-alanine and that its editing domain mainly corrects errors for other amino acids, not D-alanine.
  • The research provides biochemical evidence for chiral specificity, reinforcing that the recognition mechanisms ensure accurate protein synthesis and uphold the L-chiral bias in amino acids.
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As part of an ongoing effort to develop new antitubercular and antimicrobial agents, a series of substituted xanthenone derivatives () were synthesized. Xanthenone derivatives () were prepared a one-pot three-component thermal cyclization reaction of β-naphthol (), substituted 1-aryl-1-[1,2,3]triazole-4-carbaldehydes (), and cyclic-1,3-diones (, ) in the presence of a catalytic amount of iodine. The newly synthesized compounds were characterized by IR, NMR, mass spectral data, and elemental analysis.

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Background. TB is a global pandemic disease. All TB control programs were not successful due to the emergence of multidrug resistance in M.

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Adjuvants potentiate antigen-specific protective immune responses and can be key elements promoting vaccine effectiveness. We previously reported that the Onchocerca volvulus recombinant protein rOv-ASP-1 can induce activation and maturation of naïve human DCs and therefore could be used as an innate adjuvant to promote balanced Th1 and Th2 responses to bystander vaccine antigens in mice. With a few vaccine antigens, it also promoted a Th1-biased response based on pronounced induction of Th1-associated IgG2a and IgG2b antibody responses and the upregulated production of Th1 cytokines, including IL-2, IFN-γ, TNF-α and IL-6.

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The enzyme Necator americanus glutathione S-transferase 1 (Na-GST-1) belongs to a unique Nu class of GSTs and is a lead candidate antigen in a bivalent human hookworm vaccine. Here we describe the expression of Na-GST-1 in the yeast Pichia pastoris at the 20 L manufacturing scale and its purification process performed by three chromatographic steps, comprised of a Q Sepharose XL anion exchange column, followed by a Butyl Sepharose HP hydrophobic affinity column and a Superdex 75 size-exclusion column. Approximately 1.

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