Activation of fibrinolysis during xenoperfusion.

Ten rabbit kidneys were perfused at 80 mm Hg, 37 degrees C, with oxygenated recirculating heparinized human blood. These experiments were compared to another ten perfusion experiments where 1 unit/ml porcine plasmin was added to the human blood one hour before the perfusion, at which time the fibrinolytic activity was significant and the fibrinogen concentration under the detection limit. Rejection, determined as time until blood flow decreased to 2 ml/min, was not significantly delayed by addition of plasmin.

On the role of platelets and leucocyte in renal xenoperfusion.

Five rabbit kidneys were perfused with heparinized human blood, five with platelet-rich, leucocyte-poor blood, five with platelet-poor, leucocyte-rich blood, and five with platelet-poor, leucocyte-poor, heparinized human blood. The kidneys were perfused at a constant pressure of 80 mm Hg and the time until the flow decreased to 2 ml/min was determined. Removal of platelets from the blood significantly delayed rejection.

Xeno- and auto-perfusion of rabbit kidney. Machine perfusion with blood at 37 degrees C.

Five rabbit kidneys were perfused with human blood and another five with their own blood in a re-circulating oxygenated system at 37 degrees C. The flow decreased to 2 ml/min. within 30 min.

Antibodies against equine ALG during treatment of rejection crisis after renal allotransplantation.

The development of antibodies to equine anti-human-lymphocyte globulin (ALG) was followed using passive hemagglutination and gel diffusion, and to Forssman antigen using direct sheep erythrocyte agglutination, in a controlled clinical investigation of ALG treatment of established rejection of renal allografts in a total of 12 patients. With passive hemagglutination a weak antibody to ALG was seen only in one case. There was no increase in titer for sheep erythrocyte antigens during treatment, which agrees well with clinical observations in which no anaphylactic reactions were seen.