Monoclonal antibodies that block Roundabout 1 and 2 signaling target pathological ocular neovascularization through myeloid cells.

Roundabout (ROBO) 1 and 2 are transmembrane receptors that bind secreted SLIT ligands through their extracellular domains (ECDs) and signal through their cytoplasmic domains to modulate the cytoskeleton and regulate cell migration, adhesion, and proliferation. SLIT-ROBO signaling regulates pathological ocular neovascularization, which is a major cause of vision loss worldwide, but pharmacological tools to prevent SLIT-ROBO signaling are lacking. Here, we developed human monoclonal antibodies (mAbs) against the ROBO1 and ROBO2 ECDs.

Analytical microscopy observations of rat enterocytes after oral administration of soluble salts of lanthanides, actinides and elements of group III-A of the periodic chart.

The behavior in the intestinal barrier of nine elements (three of the group III-A, four lanthanides and two actinides), absorbed as soluble salts, has been studied by two microanalytical methods: electron probe X-ray micro analysis (EPMA) and secondary ion mass spectrometry (SIMS). It has been shown that the three elements of group III-A, aluminium, gallium and indium; and the four lanthanides, lanthanum, cerium, europium and thulium, are selectively concentrated and precipitated as non-soluble form in enterocytes of proximal part of the intestinal tract. SIMS microscopy has shown that these elements are concentrated as a number of submicroscopic precipitates, most of them localized in the apical part of the duodenum enterocytes, where they are observed from one hour to 48 hr after a single intragastric administration.

[Effects of anabolics on the thermostimulated current spectrum in calf muscles].

The effects of growth substances (zeranol, trenbolone) on calf muscle (pectoralis transversus) have been studied with a recent physical method (thermostimulated current spectroscopy). This method appears promising for detecting meat from calves treated with such hormonal substances. The authors have hypothesized that the observed differences are related to protein modification, the nature of which is yet unknown.

[Optimization of the determination of muscle acid phosphatase in the post-mortem chicken. Study of the stability of enzyme activity during meat storage at 4 degrees C. and -20 degrees C].

A colorimetric quantitative determination of total acid phosphatase in chicken muscle is proposed. Optimal conditions for the use of this enzyme were studied. This method allowed to establish that the muscular acid phosphatase of chicken does not resist to a 30 minutes incubation at 65 degrees C but that its activity is unchanged when the meat is stored at + 4 degrees C for 10 days or at -20 degrees C for 4 months.