Publications by authors named "G M Marcheggiani Muccioli"
Alterations in bile acid profile and pathways contribute to hepatic inflammation in cancer cachexia, a syndrome worsening the prognosis of cancer patients. As the gut microbiota impinges on host metabolism through bile acids, the current study aimed to explore the functional contribution of gut microbial dysbiosis to bile acid dysmetabolism and associated disorders in cancer cachexia. Using three mouse models of cancer cachexia (the C26, MC38 and HCT116 models), we evidenced a reduction in the hepatic levels of several secondary bile acids, mainly taurodeoxycholic (TDCA).
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- The study investigates how changes in bile acids contribute to metabolic dysfunction-associated steatohepatitis (MASH), focusing on the role of gut bacteria.
- Mice with MASH on a high-fat diet were compared to their wildtype counterparts to isolate the effects of MASH from diet and environmental factors.
- Findings show that MASH alters bile acid levels through mechanisms unrelated to gut microbiota, particularly highlighting increased enzyme activity in the liver that reduces secondary bile acid levels.
Ultra-endurance exercise events result in central fatigue, impacting on mental alertness and decision making. Endocannabinoids are typically elevated during endurance exercise and have been implicated in central processes such as learning and memory, but their role in central fatigue has never been studied. Twenty-four recreational male ultrarunners participated in a 100-km trail run, and 18 of them completed at least 60 km and were included in the analyses.
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- Oxysterols and bile acids are important bioactive lipids that impact various health conditions, yet tools for their simultaneous analysis are limited due to analytical difficulties.
- A new LC-MS/MS method has been developed to measure 18 oxysterols, 11 unconjugated and 15 conjugated bile acids, and 1 bile acid precursor, using isotope-labeled internal standards for accuracy.
- This method allows for the effective analysis of these lipids in both clinical and preclinical samples, successfully measuring multiple analytes from human and mouse plasma in a single run.
Article Synopsis
- - In Gram-negative bacteria, the outer membrane has a unique structure, featuring lipopolysaccharides (LPS) on the outside and glycerophospholipids (GPLs) on the inside, with the Mla system maintaining this balance by removing misplaced GPLs from the outer layer.
- - The study examines how deleting certain components affects the lipid makeup of the bacteria, changing properties like membrane stiffness and antibiotic susceptibility, while noting an overall increase in GPLs and alterations in LPS structure.
- - Understanding these lipid composition changes and their effects on the bacterial membrane can help develop new treatments for infections caused by these bacteria, which are significant in hospital settings.