Publications by authors named "G M Lacalandra"

Article Synopsis
  • Disorders of sexual development (DSDs) in dogs, especially prevalent in French Bulldogs, stem from genetic issues and breeding practices affecting genetic diversity.
  • A study on a French Bulldog with ambiguous genitalia utilized advanced genetic techniques and identified a potential connection to a known mutation linked to sex reversal syndrome, along with unusual genetic variants.
  • The results emphasize a higher level of homozygosity in the affected dog, suggesting inbreeding, and call for improved dog genome annotations and responsible breeding practices to manage genetic health.
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Article Synopsis
  • Heat stress significantly impacts canine reproductive performance and overall health, leading to increased reactive oxygen species (ROS) and sperm dysfunction.
  • A study conducted on 36 male dogs compared those exposed to heat stress (HS) with those in stable temperatures (TN) over 90 days, measuring various health parameters.
  • Results indicated HS dogs experienced decreased protein and antioxidant levels, increased ROS in sperm, and reduced semen quality compared to TN dogs, particularly noticeable after 30 days.
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The dromedary camel () fetal membranes, commonly referred to as "the placenta", are epitheliochorial, diffuse, and microcotyledonary, similarly to the mare's placenta. The evaluation of the placenta is an essential component of the neonatal evaluation in the equine species. However, post-partum or post-abortion placental assessment in dromedary camels is unfortunately too frequently neglected and, to the best of the authors' knowledge, the dromedary camel species lacks a comprehensive description of the normal placenta's gross morphology.

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Reproductive biotechnologies can be used as a supporting tool, through gamete conservation and in vitro embryo production, in the preservation of invaluable and irreplaceable animal genetic resources. In the present study, immature mouflon cumulus-oocyte complexes (COCs) collected from ovariectomized female ovaries underwent short- or long-term conservation (24 h maintained in Earle's/Hank's (EH) medium or vitrification) under field conditions and afterwards transported to the laboratory where they were cultured for in vitro maturation (IVM) and assessed for oocyte meiotic competence and bioenergetic-oxidative status. Utilization of both storage techniques led to COC morphology preservation, as well as cumulus expansion and oocyte meiotic resumption after the IVM procedure.

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