[Ultrastructure of coronary microvessels in conditions of heart reperfusion following prolonged ischemia while applying various methods of artificial hypothermia].

It has been found out that in children with Roger's disease corrected in the conditions of two fundamentally different procedures of anesthetic management, myocardial reperfusion after cardiac arrest under artificial hypothermic circulation is accompanied by obstruction of more than 30% of coronary bed microvessels with hydropic endothelial cells or their cystiform fragments. The content of necrotic cells increases, while the "working" cells demonstrate a decrease in myocropinocytotic transport characteristics. Circulatory arrest under perfusionless hypothermia and immersion reperfusion do not result in a dramatic change of general morphology of microvessels as compared to the control group, while a heterogenic response of the structures responsible for transendothelial transfer of macromolecules provides the basis for recovery of the endothelium structure and function, as a patient's temperature reaches a standard value.

The choice of the technique for harvesting and preparation of the radial artery for coronary artery bypass grafting.

At present coronary artery disease (CAD) is a most frequently occurring cause of the lethality and disability of patients. One of the basic methods for CAD treatment is coronary artery bypass grafting (CABG). Actuality of the problem of applying the radial artery (RA) for CABG is determined by the proven advantage of arterial grafts versus vein grafts in myocardial revascularization.

[Endothelial ultrastructure of myocardium microvessels affected by various methods of artificial hypothermia].

A comparative analysis of the endothelial ultrastructure of myocardium microvessels affected by various methods of artificial hypothermia was carried out. Tissue samples were harvested in children with a congenital ventricular septum defect after cooling the whole body under the conditions of hypothermic artificial circulation and perfusionless (immersion) hypothermia. It was found out that the shifts in population composition of endothelial cells, as well as the changes in the ultrastructure of organelles participating in endocellular syntheses and transendothelial transfer of macromolecules depended upon the rate body cooling.

[Endothelium of myocardium microvessel under conditions of hypothermia, ischemia, reperfusion and pharmaco-cold cardioplegia with calcium antagonist].

Microvessels of the right atrium endothelium were investigated with electron microscope for patients with congenital heart disease receiving surgical treatment under deep perfusionless hypothermia and various methods of pharmaco-cold cardioplegia. In group 1, pharmaco-cold cardioplegia was performed, with hyperosmolar normopotash solution cooled down to 2-4 degrees C. In group 2, the same solution combined with isoptin, a potash-ion blocker, was applied.

[The role of specific endothelial granules in changes of myocardium microvessel reperfusion at open-heart operations under hypothermia].

For patients with congenital heart defects, a comparative analysis was carried out of specific endothelial granules and morphological properties of perfusion capacity of microvessels at various stages of reperfusion after prolonged circulatory arrest. At all stages of reperfusion, the quantity of specific granules in microvessel endothelium dramatically dropped, as compared with the control group. At the early stages of reperfusion, the level of granule exocytosis does not lead to any statistically significant changes in perfusion characteristics in the right atrium microvessels, as compared with the control group.