Protein-Protein Interaction: Bacterial Two Hybrid.

The bacterial two-hybrid (BACTH, for "Bacterial Adenylate Cyclase-based Two-Hybrid") system is a simple and fast genetic approach to detect and characterize protein-protein interactions in vivo. This system is based on the interaction-mediated reconstitution of a cAMP signaling cascade in Escherichia coli. As BACTH uses a diffusible cAMP messenger molecule, the physical association between the two interacting chimeric proteins can be spatially separated from the transcription activation readout, and therefore, it is possible to analyze protein-protein interactions that occur either in the cytosol or at the inner membrane level as well as those that involve DNA-binding proteins.

Defining Membrane Protein Topology Using pho-lac Reporter Fusions.

Experimental determination of membrane protein topology can be achieved using various techniques. Here, we present the pho-lac dual reporter system, a simple, convenient, and reliable tool to analyze the topology of membrane protein in vivo. The system is based on the use of two topological markers with complementary properties: The Escherichia coli β-galactosidase, LacZ, which is active in the cytoplasm, and the E.

[Digital technologies in the assessment of neurological status].

Digital technologies (DT) can be used at all stages of the neurologist's work with the patient. The medical professional can obtain online information on the patient's complaints and history. DT may help to assess cognitive functions, muscular power, details of the movements including gate.

Insight into the role of the Bateman domain at the molecular and physiological levels through engineered IMP dehydrogenases.

Inosine 5'-monophosphate (IMP) dehydrogenase (IMPDH) is an ubiquitous enzyme that catalyzes the NAD -dependent oxidation of inosine 5'-monophosphate into xanthosine 5'-monophosphate. This enzyme is formed of two distinct domains, a core domain where the catalytic reaction occurs, and a less-conserved Bateman domain. Our previous studies gave rise to the classification of bacterial IMPDHs into two classes, according to their oligomeric and kinetic properties.

Hydrological bases of water resources management of the rivers of Northern Kazakhstan.

It is well known that directional global and regional climatic changes have been taking place in recent decades, in the considered area, where the underground feeding of rivers is insignificant, the river flow has decreased. In this regard, the river flow rate and total water resources of the river basins of Northern Kazakhstan are estimated in several ways: for a multi-year period, for the modern period, as well as for the period preceding it, a significant part of which is characterized by conditionally natural (not significantly transformed) river flow. Flow characteristics necessary for practice have been obtained: distribution parameters and probability values.