Connexin-43 antibodies in intraoperative diagnosis of experimental poorly differentiated gliomas.

Fluorescent diagnosis was first proposed in the early XX century and has been used in neurosurgery for about 15 years. The method relies on selective accumulation of strongly fluorescent protoporphyrin IX in tumor cells. Over the past years, the method of intraoperative fluorescence diagnosis has occupied its niche in many neurosurgical clinics around the world and is now used for fast intraoperative diagnosis in brain tumor surgery.

[Fundamental and applied aspects of the hematoencephalic barrier research].

The results of fundamental and applied studies of blood-brain barrier had been conducted by authors during the last 10 years are summarized in the publication. The molecular anatomy of barrier microvessels, as well as promising markers of BBB and other proteins involved in barrier functions are discussed. Via in vitro experiments with endothelial cells of cerebral microvessels we characterized the basic conditions required for adequate BBB modeling.

[Prospects for targeted therapy for gliomas].

This review is focused on up-to-date methods of radioimmune therapy and other approaches to the targeted treatment of high-grade gliomas. Radioimmune therapy with monoclonal antibodies to nucleoproteins, matrix and endothelial proteins and receptors of growth factors is discussed. New methods and themost promising techniques for the targeted treatment of gliomas (inactivation of signaling pathways, boron neutron capture therapy, targeted transport of nanoparticles by biomimetic protein vectors) are analysed.

[Biodegradation of 125I-labeled monoclonal antibodies after introvenous administration to rats with experimental C6 glioma].

Employment of radiolabeled antibodies in biological studies allows their specific accumulation in organs and tissues to be detected. However, stability of such labeled antibodies depends on both the method of labeling and particular experimental conditions. Therefore, stability of labeled antibodies should be determined in every particular experiment.