Publications by authors named "G M Dolganov"
A major reason that curing tuberculosis requires prolonged treatment is that drug exposure changes bacterial phenotypes. The physiologic adaptations of that survive drug exposure have been obscure due to low sensitivity of existing methods in drug-treated animals. Using the novel SEARCH-TB RNA-seq platform, we elucidated phenotypes in mice treated for with the global standard 4-drug regimen and compared them with the effect of the same regimen .
View Article and Find Full Text PDFArticle Synopsis
- Researchers developed a new RNA sequencing method called SEARCH-TB to study bacterial mRNA in the lungs during long-term tuberculosis treatment in mice.
- The study found that after 28 days of treatment with standard drugs, there was a significant suppression of genes related to bacterial growth and adaptation, indicating metabolic changes in the bacteria.
- The findings suggest that despite differences in baseline expression, the transcriptional responses in mice and bacteria during treatment were similar, highlighting the role of immune response and drug metabolism, thus showcasing SEARCH-TB as a valuable tool for improving tuberculosis treatment evaluation.
Article Synopsis
- There's a pressing need for quicker TB treatment options, as existing drugs have varying levels of effectiveness in shortening treatment duration.
- Recent research reveals that drugs can be categorized based on their effect on the synthesis of ribosomal RNA (rRNA) in the TB bacteria, Mycobacterium tuberculosis.
- By measuring rRNA precursor levels, researchers can distinguish between sterilizing and non-sterilizing drugs, which could lead to the development of more efficient drug regimens for TB treatment.
The emergence of pathogens resistant to existing antimicrobial drugs is a growing worldwide health crisis that threatens a return to the pre-antibiotic era. To decrease the overuse of antibiotics, molecular diagnostics systems are needed that can rapidly identify pathogens in a clinical sample and determine the presence of mutations that confer drug resistance at the point of care. We developed a fully integrated, miniaturized semiconductor biochip and closed-tube detection chemistry that performs multiplex nucleic acid amplification and sequence analysis.
View Article and Find Full Text PDF