β2-adrenergic regulation of T lymphocites function (in vitro study).

The aim of our study was to establish the influence of β2AR agonists and antagonists on Th1/Th2 subpopulation balance in intact and activated CD4+ T lymphocyte. Jurkat leukemic T cell line was used as a model for studying T cell activation conditions under the influence of β2AR ligands. As follows from the results of our studies, after the influence of β2AR agonist isoproterenol on intact Jurkat cells expression of IL-2 was not changed in comparison to control level.

[The role of neuroendocrine mediators in regulatory activity of T-cells].

The aim of the study was to establish the role of some neuroendocrine mediators (agonists and antagonists of β-adrenergic receptors, progesterone) in regulating T-cells activity. Studies conducted on the culture of leukemiatransformed T-cells (Jurkat cells) (DSMZ-Deutshe Sammulung von Mikroorganismen und Zellkulturen (Germany)). Jurkat Cells (4 x 105 cells/ml) stimulated with 50 µg/ml fitogemaglutinin A (PHA) at 370С for 5 minutes and then incubated for 24 hours alone, or together with β- adrenergic receptors agonist izopretonolom (at dose of 10-5 M, 10-6 M), an antagonist, propranolol (dose of 10-5 M, 10-6 M) and progesterone (dose 0,07 µl, 0,7 µl) added to the incubation medium.

Alteration in viability and proliferation activity of mitogen stimulated Jurkat cells.

The aim of our study was the establishment of mechanisms of alterations in viability and mitogen stimulated T lymphocyte proliferation activity. In PHA-stimulated Jurkat cells along with increased proliferation level the reduction of mitochondrial dehydrogenases activity (by 20%) and rising number of cells with low mitochondrial membrane potential (ΔΨm) was revealed. It was concluded that interaction of mitogen (PHA) with T cells receptors (TCR) contributes reduction activity of mitochondrial NADH-dehydrogenase (via phospolipasa C-diacilglicerol (PLC-DAG)-induced PKCs (PKCα, PKCθ) activation pathway).

Age related alterations of adrenoreceptor activity in erythrocyte membrane.

The aim of the study was the investigation of age-related functional alterations of adrenoreceptors and the effect of agonist and antagonist drugs on age related adrenoreceptor activity in erythrocyte membrane. The impact of isopropanol and propanol on functional activity β- adrenergic receptors in red blood cell membrane were studied in 50 practically healthy men--volunteers. (I group--75-89 years old, II group--22-30 years old).

Deformability of red blood cells and human aging.

The goal of the research was to study the mechanisms of the regulation of deformability of RBCs in various aged people. Morphological, biophysical and biochemical parameters of RBCs were studied in 50 practically healthy men - volunteers (divided in two age groups: adults (22-30 years old), and old people (75-89 years old)). It was conclude that excess number of large-sized matured (aged) erythrocytes is revealed in the blood of old people.